The effects of platelet lysate on maturation, fertilization and embryo development of NMRI mouse oocytes at germinal vesicle stage
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چکیده
منابع مشابه
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
the effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...
متن کاملP-29: Effects of Cumulus Cells on Survival, In vitro Maturation,Fertilization and Subsequent Developmental Capacity of Mouse Germinal Vesicle Stage Oocytes during Vitrification
Background: Cryopreservation of oocytes, which is an interesting procedure to conserve female gametes, is an essential part of reproductive biotechnology. The objective of the present study was to investigate the effects of cumulus cells on survival,in vitro maturation,fertilization and subsequent developmental capacity of mouse germinal vesicle stage oocytes during vitrification. Materials and...
متن کاملthe effect of retinoic acid on in vitro maturation and fertilization rate of mouse germinal vesicle stage oocytes
objective: retinoids are recognized as important regulators of cell differention and tissue function. previous studies, performed both in vivo and in vitro, indicate that retinoids influence several reproductive events. in this study, we investigated the effect of all-trans retinoic acid (t-ra) on maturation and fertilization rate of immature oocytes (germinal vesicle). materials and methods: g...
متن کاملThe Effect of Progesterone on the In vitro Maturation and Developmental Competence of Mouse Germinal Vesicle Oocytes
a:4:{s:10:"Background";s:206:"The aim of the present study was to investigate the role of progesterone on the developmental competence of cumulus-oocyte complexes (COCs) and cumulus-denuded oocytes (CDOs) at germinal vesicle (GV) stage.";s:19:"MaterialsAndMethods";s:485:"GV oocytes of pregnant mare's serum gonadotropin (PMSG)-primed prepubertal mice were divided into two groups: CDOs and COCs. ...
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ژورنال
عنوان ژورنال: Reproductive Medicine and Biology
سال: 2015
ISSN: 1445-5781
DOI: 10.1007/s12522-015-0220-x